| Catalog# |
C040 |
| Source |
P. pastoris |
| Description |
Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor/GM-CSF is produced with our Pichia pastoris expression system. The target protein is expressed with sequence (Ala18-Glu144) of Human GM-CSF. |
| Names |
Granulocyte-Macrophage Colony-Stimulating Factor, GM-CSF, Colony-Stimulating Factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF |
| Accession # |
P04141 |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 10mM Tris-HCl, 4% Mannitol, 1% Sucrose, pH 8.5 |
| Shipping |
The product is shipped at ambient temperature. |
| Reconstitution |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Biological Activity |
ED50 is less than 0.2 ng/ml as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
Specific Activity of 5.0 x 106 IU/ mg. |
| Purity |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Endotoxin |
Less than 0.1 ng/μg (1 IEU/μg). |
| Amino Acid Sequence |
APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQG LRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
|
| Background |
GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages, and eosinophils, GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. |
