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C276
Recombinant Human MDH1/Malate dehydrogenase, cytoplasmic
10ug
1200
1080
现货
国产
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C276
Recombinant Human MDH1/Malate dehydrogenase, cytoplasmic
50ug
3520
3168
现货
国产
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C276
Recombinant Human MDH1/Malate dehydrogenase, cytoplasmic
500ug
12320
11088
现货
国产
-
C276
Recombinant Human MDH1/Malate dehydrogenase, cytoplasmic
1mg
17600
15840
现货
国产
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Catalog# C276 Source E.coli Description Recombinant Human Malate Dehydrogenase, Cytoplasmic/MDH1 is produced by our E. coli expression system. The target protein is expressed with sequence (Ser2-Ala334) of Human MDH1 fused with a 6His tag at the C-terminus. Names Malate Dehydrogenase Cytoplasmic, Cytosolic Malate Dehydrogenase, Diiodophenylpyruvate Reductase, MDH1, MDHA Accession # P40925 Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0 Shipping The product is shipped on dry ice/ice packs. Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLL KDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVG NPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDV NHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFG TPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKE SAFEFLSSALEHHHHHHBackground Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.