| Catalog# |
C239 |
| Source |
E.coli |
| Description |
Recombinant Human Neurocalcin-D/NCALD is produced by our E. coli expression system. The target protein is expressed with sequence (Gly2-Phe193) of Human NCALD fused with a His tag at the N-terminus. |
| Names |
Neurocalcin-Delta, NCALD |
| Accession # |
P61601 |
| Formulation |
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 40% Glycerol, pH 8.0 |
| Shipping |
The product is shipped on dry ice/ice packs. |
| Storage |
Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. |
| Purity |
Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin |
Less than 0.1 ng/μg (1 IEU/μg). |
| Amino Acid Sequence |
MGSSHHHHHHSSGLVPRGSHMGKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLSM EEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDL DGNGYISKAEMLEIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNRDGKLSLEEFIRGAKS DPSIVRLLQCDPSSAGQF
|
| Background |
Neurocalcin-delta (NCALD) is a neuronal calcium-binding protein that belongs to the neuronal calcium sensor (NCS) family. It expressed in mammalian brains. NCALD contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein possesses a Ca2+ /myristoyl switch. It is cytosolic at resting calcium levels. However, elevated intracellular calcium levels induce a conformational change which exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. NCALD protein is thought to be a regulator of G protein-coupled receptor signal transduction. |
