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C055
Recombinant Human CXCL4/PF4
10ug
936
842.4
现货
国产
-
C055
Recombinant Human CXCL4/PF4
50ug
2800
2520
现货
国产
-
C055
Recombinant Human CXCL4/PF4
500ug
9840
8856
现货
国产
-
C055
Recombinant Human CXCL4/PF4
1mg
14080
12672
现货
国产
-
C055
Recombinant Human PF4/CXCL4
10ug
936
842.4
现货
国产
-
C055
Recombinant Human PF4/CXCL4
50ug
2800
2520
现货
国产
-
C055
Recombinant Human PF4/CXCL4
500ug
9840
8856
现货
国产
-
C055
Recombinant Human PF4/CXCL4
1mg
14080
12672
现货
国产
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Catalog# C055 Source E.coli Description Recombinant Human C-X-C Motif Chemokine Ligand 4/CXCL4 produced in E. coli is a single non-glycosylated polypeptide chain containing 70 amino acids with a molecular mass of 7.8 kD. Names Platelet Factor 4, PF-4, C-X-C Motif Chemokine 4, Iroplact, Oncostatin-A, PF4, CXCL4, SCYB4 Accession # P02776 Formulation Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, pH 8.0 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Biological Activity ED50 is 5-15 μg/mL as determined by the ability of Recombinant CXCL4 to inhibit human FGF basic dependent proliferation of NR6R3T3 mouse fibroblasts. Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence EAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIK KLLESBackground Human Chemokine (C-X-C motif) Ligand 4 (CXCL4) is expressed in megakaryocytes and stored in the alpha-granules of platelets. CXCL4 contains several heparin-binding sites at the C-terminal region and binds heparin with high affinity. The active CXCL4 protein is a tetramer. Human and mouse CXCL4 share 64% sequence identity. CXCL4 is chemotactic for neutrophils, fibroblasts and monocytes and plays a critical role in inflammation and wound repair. CXCL4 functions via a splice variant of the chemokine receptor CXCR3, known as CXCR3B. The major physiologic role of CXCL4 appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes.