| Catalog# |
CB69 |
| Source |
HEK293 |
| Description |
Recombinant Human Semaphorin-4B/SEMA4B is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu39-Glu712) of Human SEMA4B fused with a polyhistidine tag at the C-terminus. |
| Names |
Semaphorin-4B, also known as KIAA1745, SEMAC and SEMA4B, is a single-pass type I membrane protein. SEMA4B is a member of the semaphorin family. |
| Accession # |
Q9NPR2 |
| Formulation |
Supplied as a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4 |
| Shipping |
The product is shipped on dry ice/ice packs. |
| Storage |
Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. |
| Purity |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Endotoxin |
Less than 0.1 ng/µg (1 IEU/µg). |
| Amino Acid Sequence |
LSPRISLPLGSEERPFLRFEAEHISNYTALLLSRDGRTLYVGAREALFALSSNLSFLPGGEYQEL LWGADAEKKQQCSFKGKDPQRDCQNYIKILLPLSGSHLFTCGTAAFSPMCTYINMENFTLARDEK GNVLLEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDPAISRSQSLRPTKTESSLNWLQDP AFVASAYIPESLGSLQGDDDKIYFFFSETGQEFEFFENTIVSRIARICKGDEGGERVLQQRWTSF LKAQLLCSRPDDGFPFNVLQDVFTLSPSPQDWRDTLFYGVFTSQWHRGTTEGSAVCVFTMKDVQR VFSGLYKEVNRETQQWYTVTHPVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQV RSRMLLLQPQARYQRVAVHRVPGLHHTYDVLFLGTGDGRLHKAVSVGPRVHIIEELQIFSSGQPV QNLLLDTHRGLLYAASHSGVVQVPMANCSLYRSCGDCLLARDPYCAWSGSSCKHVSLYQPQLATR PWIQDIEGASAKDLCSASSVVSPSFVPTGEKPCEQVQFQPNTVNTLACPLLSNLATRLWLRNGAP VNASASCHVLPTGDLLLVGTQQLGEFQCWSLEEGFQQLVASYCPEVVEDGVADQTDEGGSVPVII STSRVSAPAGGKASWGADRSYWKEVDHHHHHH*
|
| Background |
The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. SEMA4B is a single-pass type I membrane protein and contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one Sema domain. Human SEMA4B is expressed in neurons. SEMA4B inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. SEMA4B negatively regulates basophil-mediated Th2 and humoral memory responses. |
