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C445
Recombinant Human CD33/Siglec-3
10ug
640
576
现货
国产
-
C445
Recombinant Human CD33/Siglec-3
50ug
1760
1584
现货
国产
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C445
Recombinant Human CD33/Siglec-3
500ug
12320
11088
现货
国产
-
C445
Recombinant Human CD33/Siglec-3
1mg
17600
15840
现货
国产
-
C445
Recombinant Human Siglec-3/CD33
10ug
640
576
现货
国产
-
C445
Recombinant Human Siglec-3/CD33
50ug
1760
1584
现货
国产
-
C445
Recombinant Human Siglec-3/CD33
500ug
12320
11088
现货
国产
-
C445
Recombinant Human Siglec-3/CD33
1mg
17600
15840
现货
国产
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Catalog# C445 Source HEK293 Description Recombinant Human CD33 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Asp18-His259) of Human CD33 with a C-terminal Fc fusion tag and His tag. Names Myeloid Cell Surface Antigen CD33, Sialic Acid-Binding Ig-Like Lectin 3, Siglec-3, gp67, CD33, SIGLEC3 Accession # P20138 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 2mM EDTA, pH 7.2 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence DPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEV QEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKI LIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTC QVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVHTSDIEGRMDEPKSCDKTH TCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKT KPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVS?NKALPAPIEKTISKAKGQPREPQVYTLPP SREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQ QGNVFSCSVMHEALHNHYTQKSLSLSPGKVDHHHHHHBackground CD33 is a type I Lectin belonging to the Ig superfamily. CD33 contains an N terminal Ig like V type domain, which mediates sialic acid binding, followed by one Ig like C2 type domain, a transmembrane region and a cytoplasmic tail containing two conserved immunoreceptor tyrosine based inhibition motifs (ITIMs). Eleven human Siglecs have been characterized. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec3 both in their extracellular and intracellular regions. They are collectively referred to as CD33 related Siglecs. CD33 related Siglecs have differential expression pattern within the hematopoietic system. They are involved in the regulation of cellular activation within the immune system. Siglec 3 expression is restricted to cells of myelomonocytic lineage. Siglec3 recruits SHP1 and SHP2 to its ITIMs upon phosphorylation.