产品中心
-
产品货号
产品名称
规格
销售价
会员价
货期
产地
订购
-
C541
Recombinant Human SP-D/Pulmonary surfactant-associated protein D
10ug
640
576
现货
国产
-
C541
Recombinant Human SP-D/Pulmonary surfactant-associated protein D
50ug
1760
1584
现货
国产
-
C541
Recombinant Human SP-D/Pulmonary surfactant-associated protein D
500ug
12320
11088
现货
国产
-
C541
Recombinant Human SP-D/Pulmonary surfactant-associated protein D
1mg
17600
15840
现货
国产
- 产品简介
- 使用说明
- 相关产品
- 参考文献
- 购买咨询
Catalog# C541 Source HEK293 Description Recombinant Human Pulmonary Surfactant-Associated Protein D/SP-D produced by transfected human cells is a secreted protein with sequence (Ala21-Phe375) of Human SFTPD fused with a polyhistidine tag at the C-terminus. Names Pulmonary Surfactant-Associated Protein D, PSP-D, SP-D, Collectin-7, Lung Surfactant Protein D, SFTPD, COLEC7, PSPD, SFTP4 Accession # P35247 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence AGMKTYSHRTMPSACTLVMCSSVESGLPGRDGRDGREGPRGEKGDPGLPGAAGQAGMPGQAGPVG PKGDNGSVGEPGPKGDTGPSGPPGPPGVPGPAGREGPLGKQGNIGPQGKPGPKGEAGPKGEVGAP GMQGSAGARGLAGPKGERGVPGERGVPGNTGAAGSAGAMGPQGSPGARGPPGLKGDKGIPGDKGA KGESGLPDVASLRQQVEALQGQVQHLQAAFSQYKKVELFPNGQSVGEKIFKTAGFVKPFTEAQLL CTQAGGQLASPRSAAENAALQQLVVAKNEAAFLSMTDSKTEGKFTYPTGESLVYSNWAPGKPNDD GGSEDCVEIFTNGKWNDRACGEKRLVVCEFVDHHHHHHBackground Surfactant Pulmonary-Associated Protein D (SP-D) is a 43 kDa member of the collectin family of innate immune modulators. Its principal components consist of a collagen-like region and a C-terminal carbohydrate recognition domain (CRD), a structure that places it in a subset of pattern recognition proteins termed defense collagens. SP-D is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures and induced in cardiac smooth muscle and endothelial cells. It binds both secreted and transmembrane proteins that transduce its function. It binds human neutrophil defensins, modulating influenza anti-viral defense. It binds MD-2/LY96, a secreted protein that cooperates with Toll-like receptors (TLRs) in the response of macrophages to bacterial lipopolysaccharides (LPS) or cell wall components. It also binds macrophage CD14 and TLRs directly, blocking binding of LPS and down-regulating TNF-α secretion. SP-D binding of both SIRPα and the calreticulin/CD91 complex on macrophages allows for a graded response to environmental challenge via the following mechanism: when the ratio of antigen/pathogen to available CRDs is low, antigen can be bound without occupying all available CRDs. The free CRDs will bind to SIRPα, generating a signal that downmodulates the inflammatory response. When virtually all CRDs are occupied by ligand, however, free CRDs are not available for SIRPα binding. Instead, the dodecamer is thought to rearrange, exposing the N-termini of all four linked trimers which bind to the calreticulin/CD91 complex and initiate inflammation. Thus, SP-D provides a mechanism for the clearance of small antigenic insults without the need for a damaging inflammatory response.