| Catalog# |
C182 |
| Source |
E.coli |
| Description |
Recombinant Human Thioredoxin-2/TXN2 is produced with our E. coli expression system. The target protein is expressed with sequence (Thr60-Gly166) of Human TXN2. |
| Names |
Thioredoxin Mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2 |
| Accession # |
Q99757 |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4 |
| Shipping |
The product is shipped at ambient temperature. |
| Reconstitution |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Purity |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Endotoxin |
Less than 0.1 ng/μg (1 IEU/μg). |
| Amino Acid Sequence |
MTTFNIQDGPDFQDRVVNSETPVVVDFHAQWCGPCKILGPRLEKMVAKQHGKVVMAKVDIDDHTD LAIEYEVSAVPTVLAMKNGDVVDKFVGIKDEDQLEAFLKKLIG
|
| Background |
Thioredoxin-2 (TXN2) is a mitochondrial member of the thioredoxin family. Thioredoxin-2 is extensively expressed in adult and fetal tissues. Thioredoxin-2 contains an N-terminal 59 amino acid transit peptide, which is cleaved before translocating to mitochondria. Mitochondrial thioredoxin play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Thioredoxin-2 could be involved in the resistance to anti-tumor agents and possesses a dithiol-reducing activity. In addition, Thioredoxin-2 is important at low oxidative stress conditions. |
